The bioremediation potential for a consortium including two bacteria (Pseudomonas sp and Serratia marcescens), two fungi (Aspergillus sp and Trichoderma sp) and inorganic fertiliser incorporated into the soil, and the phytoremediation potential of the Para grass (Brachiaria mutica) and the leguminous "guaje" (Leucaena leucocephala), were evaluated both in a soil polluted with fresh crude oil (PN) recently incorporated into the soil, and in a soil with 25-year old weathered crude oil (PI) from chronic oil spills. The bioassay was 4x3x2x2 factorial: with the oil [150 mg kg-1 total oil hydrocarbons (HTP) from biogenic sources, 50 000 and 78 000 mg kg-1 HTP of PN and 79 457 mg kg-1 HTP of PI], the plant (without plant, with Para grass or with guaje), the microbial consortium (without and with inoculum, 5 mL of bacterial inoculum with 250x10(6) CFU mL-1 + 40x10(5) fungal CFU mL-1) and the fertiliser (without and with fertiliser, 160-50-00 dose). Forty eight treatments were evaluated. Quantified at the start (day 1) and end (day 158) were the degradation of the HTP, and the bacteria and fungi populations. The measurement of bacteria and fungi followed the counting method in Petri dishes. HTP extraction was carried out with dichloromethane according to the EPA 418.1 method, and quantification by gravimetry followed the NMX-AA-134-SCFI-2006. The interaction among the rhizosphere, the fertiliser and the microbial consortium generated significant positive effects (Tukey p < 0.05) and promoted new oil degradation and weathering. The greatest degradation after 158 days was of 47% in the soil with 50 000 mg kg-1 of PN, and the lowest was that in soils with 78 000 and 79 457 mg kg-1 of PN and PI respectively. The leguminous guaje inoculated with the consortium and fertiliser has a great potential for use in phytoremediation of soils with 50 000 mg kg-1 of HTP and PN. The degradation of HTP was promoted up to 81%, 9% more than in the Egypt grass rhizosphere. In soils with PI, the Egypt grass was the best with a degradation of 44%.

Se evaluó el potencial de un consorcio microbiano constituido por bacterias (Pseudomonas sp y Serratia marcescens y hongos (Aspergillus sp y Trichoderma sp) y de fertilizante inorgánico incorporados al suelo en la biorremediación, y el potencial del pasto egipto (Brachiaria mutica) y de la leguminosa guaje (Leucaena leucocephala) en la fitorremediación de un suelo contaminado con petróleos nuevo (PN) recién incorporado al suelo y el intemperizado (PI) procedente de derrames crónicos de petróleo desde hace 25 años. El experimento fue factorial 4x3x2x2, estos fueron petróleo [150 mg kg-1 de hidrocarburos totales del petróleo (HTP) de origen biogénico, 50 000 y 78 000 mg kg-1 HTP de PN y 79 457 mg kg-1 HTP de PI], planta (sin planta, con planta de pasto egipto o de guaje), consorcio microbiano (sin inóculo y con inóculo, 5 mL de inóculo bacteriano con 250x10(6) UFC mL-1 + 40x10(5) UFC de hongos mL-1) y fertilizante (sin fertilizante y con fertilizante, dosis 160-50-00). Se evaluaron 48 tratamientos. Se cuantificaron al inicio (día 1) y al final (día 158) la degradación de los HTP y las poblaciones de bacterias y hongos. La medición de bacterias y hongos se realizó con el método de recuento en cajas Petri. La extracción de HTP se efectuó con diclorometano, procedimiento EPA 418.1 y la cuantificación por gravimetría según la NMX-AA-134-SCFI-2006. La interacción de rizósfera, consorcio microbiano y el fertilizante originaron efectos positivos significativos (Tukey p < 0.05), promovieron la degradación del PN y el PI. La mayor degradación a los 158 d fue 47% en el suelo con 50 000 mg kg-1 de PN y la menor en suelos con 78 000 y 79 457 mg kg-1 de PN y PI, respectivamente. La leguminosa guaje inoculada con consorcio y fertilizante tiene un amplio potencial de uso en la fitorremediación de un suelo con 50 000 mg kg-1 de HTP de PN, se promovió la degradación de HTP hasta un 81 %, 9% más respecto a la rizósfera del pasto Egipto, pero en suelo con PI el mejor fue en pasto Egipto con un 44% de degradación.

The densities of free living total N-fixing bacteria in soil down to a depth of 110 cm and in rhizospheric soils of banana plants established in a eutric Fluvisol, were evaluated. Soil samples were collected from the six horizons of the profile and from 30 rhizospheric sampling points (0-10 cm depth) randomly distributed throughout 10 000 m ². Populations of Azospirillum and Azotobacter were isolated at depths of 59 and 110 cm, respectively. The largest isolates were 42x10(4) and 11x10(5) CFU g-1 of dry soil for Azotobacter and Azospirillum, respectively. Three isolates of Azospirillum and Azotobacter each were used in the formulation of sustained microbial inoculants in organic media (chicken manure, chicken manure + soil, rachis of banana raceme, rachis of banana raceme + soil) and in control soil. Two integrated bioassays with 10 treatments each were established for 60 days of incubation. Densities were counted on days 1, 30 and 60, and total nitrogen was quantified on day 60. Azospirillum grew best on rachis of banana raceme + consortium with 15x10(5) CFU per gram of biofertilizer, whereas Azotobacter grew best on 63x10(4) CFU per gram of the biofertilizer formulated with rachis of banana raceme + soil + consortium. The greatest total nitrogen content was 5.25% released into the soil with chicken manure and bioaugmented with the Azospirillum + Azotobacter consortium. However, the Azospirillum and Azotobacter populations decreased to 68x10(4) and 20x10(4) CFU g-1 of biofertilizer, respectively.

Se determinaron las densidades de bacterias totales fijadoras de N de vida libre en suelo hasta 110 cm de profundidad y en suelo rizosférico de la planta de banano establecido en un Fluvisol éutrico. Se colectaron muestras de suelo en los seis horizontes del perfil y en 30 puntos de muestreo rizosférico (0-10 cm de profundidad) distribuidos en forma aleatoria en 10 000 m². Las poblaciones de Azospirillum y Azotobacter se aislaron hasta profundidades de 59 y 110 cm, respectivamente. Los gremios aislados más grandes fueron 42x10(4) y 11x10(5) UFC g-1 de suelo seco para Azospirillum y Azotobacter, respectivamente. Tres aislados de Azospirillum y tres de Azotobacter se utilizaron en la formulación de inoculantes microbianos sostenidos en soportes orgánicos (pollinaza, pollinaza + suelo, pinzote, pinzote + suelo) y en suelo testigo. Se establecieron dos bioensayos integrados con 10 tratamientos cada uno durante 60 días de incubación. Las densidades se contaron los días 1, 30 y 60, y el nitrógeno total se cuantificó el día 60. Azospirillum creció mejor en pinzote + inóculo con 15x10(5) UFC por cada gramo de biofertilizante mientras que para Azotobacter fue 63x10(4) UFC en pinzote + suelo + inóculo. El mayor contenido de nitrógeno total fue 5.25%, fue liberado en el suelo con pollinaza y bioaumentado con el consorcio Azospirillum + Azotobacter; no obstante, las poblaciones de Azospirillum y Azotobacter disminuyeron a 68x10(4) y 20x10(4) UFC g-1 de biofertilizante, respectivamente.

OBJETIVO: Identificar o perfil de mulheres com incontinência urinária (IU) atendidas em um serviço público de Fisioterapia Uroginecológica, em relação a características sociodemográficas e clínicas. MATERIAIS E MÉTODOS: Neste estudo descritivo transversal retrospectivo, por meio de prontuários e fichas de avaliação fisioterapêutica das participantes, os seguintes dados foram levantados: idade, estado civil, grau de instrução, tipo de incontinência, fatores de risco, sinais e sintomas, função perineal (escala de Oxford) e qualidade de vida (IQoL). Estatística descritiva, pela distribuição de freqüência e proporção, foi aplicada. RESULTADOS: Dados de 58 participantes foram considerados. A maioria tinha idade entre 40 e 59 anos (81%), era casada (62%) e possuía grau de instrução fundamental (79%). A IU mista foi prevalente em 63% da amostra e a incontinência urinária de esforço (IUE) em 34%. Gestações (88%) e partos vaginais (76%) se destacaram como fatores de risco e o sintoma mais prevalente foi perda de urina ao esforço (97%). O grau 2 de função perineal foi o mais freqüente (41%) e a distribuição da qualidade de vida das participantes variou entre baixa (10%), moderada (33%), boa (28%) e ótima (24%). CONCLUSÕES: Este estudo oferece dados que contribuem para o conhecimento do perfil das mulheres com IU atendidas em serviços públicos que prestam assistência fisioterapêutica uroginecológica e, além disso, poderá auxiliar no desenvolvimento de intervenções preventivas e reabilitadoras nestes serviços.

OBJECTIVE: To identify the sociodemographic and clinical profile of women with urinary incontinence attended at a public urogynecological physical therapy service. METHODS: In this retrospective cross-sectional descriptive study, the following information were gathered from the participants’ hospital records and physical therapy evaluation forms: age, marital status, educational level, type of incontinence, risk factors, signs and symptoms, perineal function (Oxford scale) and quality of life (QOL). Descriptive statistics using frequency distributions and proportions were applied. RESULTS: Data from 58 participants were considered. Most of them were between 40 and 59 years old (81%), were married (62%) and only had elementary education (79%). Mixed urinary incontinence was the most prevalent type (63%), followed by stress urinary incontinence (34%). Pregnancy (88%) and vaginal delivery (76%) were the most prevalent risk factors and the most prevalent symptom was urinary loss under stress (97%). Perineal function grade 2 was the most frequent type (41%) and the participants’ quality of life distribution ranged between poor (10%), moderate (33%), good (28%) and excellent (24%). CONCLUSIONS: This study provides data that contribute towards ascertaining the profile of the women with urinary incontinence who are attended in public services that offer urogynecological physical therapy. Furthermore, it may assist in developing preventive and rehabilitative interventions in such services.